Protein Purification Reagents and Kits
STEMCELL Technologies RoboSep™ Human Myeloid DC Enrichment Kit, For processing: 2 x 10^9 cells
The EasySep™ Human Myeloid DC Enrichment Kit is designed to isolate myeloid dendritic cells (mDC) from fresh peripheral blood mononuclear cells or ammonium chloride-lysed leukapheresis by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-mDCs and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Alkali Scientific LB Agar (Miller) w/Ampicillin & Chloramphenicol 20mL Plate, 10/Pk
LB Agar (Miller) with Ampicillin and Chloramphenicol is a dual selection medium designed for molecular biology workflows requiring maintenance of two resistance markers. Each plate contains 20 mL of high salt Miller formulation Lysogeny Broth agar supplemented with both antibiotics. This medium is commonly used in co transformation experiments where a single E. coli cell must maintain two compatible plasmids, such as an expression vector and a reporter plasmid. It is also suitable for specialized strains like BL21(DE3)pLysS or Rosetta that carry helper plasmids conferring chloramphenicol resistance. Ampicillin maintains plasmid selection while chloramphenicol ensures retention of the helper plasmid, allowing stable propagation of both genetic elements during cloning and protein expression experiments.
STEMCELL Technologies RosetteSep™ Human Granulocyte Depletion Cocktail, For processing: 200 mL whole blood processed
The RosetteSep™ Human Granulocyte Depletion Cocktail is designed to deplete granulocytes from whole blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The granulocyte-depleted fraction is present as a highly enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies EasySep™ Mouse CD4 Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD4 Positive Selection Kit II is designed to isolate CD4+ cells from single-cell suspensions of splenocytes or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD4 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse CD4 Positive Selection Kit (Catalog #18752) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
Miltenyi Biotec, Inc. CD62L pure, mouse
Detection of mouse L-selectin–expressing cells by mass cytometry.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Miltenyi Biotec, Inc. MACSxpress WB Eosinophil Iso. Kit, h
Isolation of untouched human eosinophils from freshly drawn anticoagulated whole blood without density gradient centrifugation
Abcam Human Platelet Isolation Kit
Human Platelet Isolation Kit
The product is subject to the following: Abcam Restricted Use Statement
STEMCELL Technologies RosetteSep™ Human CD4+ T Cell Enrichment Cocktail, For processing: 40 mL whole blood processed
The RosetteSep™ Human CD4+ T Cell Enrichment Cocktail is designed to isolate CD4+ T cells from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD4+ T cells and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified CD4+ T cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
Miltenyi Biotec, Inc. CD45RA-APC
T6D11 reacts with human CD45RA. The fluorescently labeled CD45RA antigen can be detected by flow cytometry..
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Biolegend APC anti-mouse Ly-6A/E Sca-1 R
APC anti-mouse Ly-6A/E (Sca-1) Recombinant [QA17A36]; Isotype: Rat IgG1, κ; Reactivity: Mouse; Apps: FC; Size: 100 μg
Miltenyi Biotec, Inc. MZ and FO B Cell Isolation Kit, mouse
Isolation of marginal zone (MZ) B cells and follicular (FO) B cells from mouse spleen.
STEMCELL Technologies EasySep™ Magnet, For isolating: 1 Unit
The EasySep™ Magnet is designed for cell separation procedures using EasySep™ reagents. The EasySep™ Magnet generates a high-gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with EasySep™ Magnetic Particles without the use of columns. This magnet is designed to hold a standard 12 x 75 mm (5 mL) polystyrene tube.